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The forward and reverse primers for real-time polymerase chain reaction

Journal: Neural Regeneration Research

Article Title: Nerve root magnetic stimulation regulates the synaptic plasticity of injured spinal cord by ascending sensory pathway

doi: 10.4103/NRR.NRR-D-24-00628

Figure Lengend Snippet: The forward and reverse primers for real-time polymerase chain reaction

Article Snippet: The following antibodies were used: postsynaptic density protein-95 (PSD95; rabbit, 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 3450S, RRID: AB_2292883), Synapsin I (rabbit, 1:1000, Abmart, Shanghai, China, Cat# 334286), GAP43 (mouse, 1:400, Santa Cruz, Biotechnology, Dallas, TX, USA, Cat# sc-17790, RRID: AB_627660), Synapsin II (rabbit, 1:1000, Abclonal, Wuhan, China, Cat# A19542), and GAPDH (mouse, 1:20 000, Sigma-Aldrich, Cat# G8795, RRID: AB_1078991).

Techniques:

NRMS upregulates transcriptional levels of genes related to synaptic plasticity in damaged region of SCI rats. (A) The functional classification of different genes in the SCI + NRMS and SCI + SS groups by GO enrichment analysis. (B) The functional classification of different genes between the SCI + NRMS and SCI + SS groups by KEGG database. (C–F) The relative levels of Syn2 , Syp , GAP43 , and MAP2 mRNA normalized by the Sham group. The bold lines, upper boundaries, and lower boundaries represent the medians, 75 th percentiles, and 25 th percentiles, respectively. Whiskers extend 1.5 times interquartile range ( n = 4 per group). * P < 0.05, vs . Sham group; # P < 0.05, vs . SCI + SS group. GAP43: Growth-associated protein 43; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; MAP2: microtubule-associated protein 2; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation; Syn2: synapsin II; Syp: synaptophysin.

Journal: Neural Regeneration Research

Article Title: Nerve root magnetic stimulation regulates the synaptic plasticity of injured spinal cord by ascending sensory pathway

doi: 10.4103/NRR.NRR-D-24-00628

Figure Lengend Snippet: NRMS upregulates transcriptional levels of genes related to synaptic plasticity in damaged region of SCI rats. (A) The functional classification of different genes in the SCI + NRMS and SCI + SS groups by GO enrichment analysis. (B) The functional classification of different genes between the SCI + NRMS and SCI + SS groups by KEGG database. (C–F) The relative levels of Syn2 , Syp , GAP43 , and MAP2 mRNA normalized by the Sham group. The bold lines, upper boundaries, and lower boundaries represent the medians, 75 th percentiles, and 25 th percentiles, respectively. Whiskers extend 1.5 times interquartile range ( n = 4 per group). * P < 0.05, vs . Sham group; # P < 0.05, vs . SCI + SS group. GAP43: Growth-associated protein 43; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; MAP2: microtubule-associated protein 2; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation; Syn2: synapsin II; Syp: synaptophysin.

Article Snippet: The following antibodies were used: postsynaptic density protein-95 (PSD95; rabbit, 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 3450S, RRID: AB_2292883), Synapsin I (rabbit, 1:1000, Abmart, Shanghai, China, Cat# 334286), GAP43 (mouse, 1:400, Santa Cruz, Biotechnology, Dallas, TX, USA, Cat# sc-17790, RRID: AB_627660), Synapsin II (rabbit, 1:1000, Abclonal, Wuhan, China, Cat# A19542), and GAPDH (mouse, 1:20 000, Sigma-Aldrich, Cat# G8795, RRID: AB_1078991).

Techniques: Functional Assay

NRMS alters expression of proteins associated with synaptic plasticity in the injured spinal cord of rats. (A) Representative western blots show the expression levels of PSD95, GAP43, Synapsin I and Synapsin II collected from the damaged area of spinal cord on day 22 after SCI. (B–E) The semi-quantified results of PSD95, GAP43, Synapsin I, and Synapsin II protein levels. All experiments were repeated three times. Data are expressed as mean ± SD ( n = 5 per group). ** P < 0.01, *** P < 0.001, vs. Sham group; # P < 0.05, ## P < 0.01, vs . SCI + SS group. GAP43: Growth-associated protein 43; PSD95: postsynaptic density protein-95; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation.

Journal: Neural Regeneration Research

Article Title: Nerve root magnetic stimulation regulates the synaptic plasticity of injured spinal cord by ascending sensory pathway

doi: 10.4103/NRR.NRR-D-24-00628

Figure Lengend Snippet: NRMS alters expression of proteins associated with synaptic plasticity in the injured spinal cord of rats. (A) Representative western blots show the expression levels of PSD95, GAP43, Synapsin I and Synapsin II collected from the damaged area of spinal cord on day 22 after SCI. (B–E) The semi-quantified results of PSD95, GAP43, Synapsin I, and Synapsin II protein levels. All experiments were repeated three times. Data are expressed as mean ± SD ( n = 5 per group). ** P < 0.01, *** P < 0.001, vs. Sham group; # P < 0.05, ## P < 0.01, vs . SCI + SS group. GAP43: Growth-associated protein 43; PSD95: postsynaptic density protein-95; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation.

Article Snippet: The following antibodies were used: postsynaptic density protein-95 (PSD95; rabbit, 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 3450S, RRID: AB_2292883), Synapsin I (rabbit, 1:1000, Abmart, Shanghai, China, Cat# 334286), GAP43 (mouse, 1:400, Santa Cruz, Biotechnology, Dallas, TX, USA, Cat# sc-17790, RRID: AB_627660), Synapsin II (rabbit, 1:1000, Abclonal, Wuhan, China, Cat# A19542), and GAPDH (mouse, 1:20 000, Sigma-Aldrich, Cat# G8795, RRID: AB_1078991).

Techniques: Expressing, Western Blot